Course registration fee : We have special rates for early registerations. Details are below.

All registrations made by May 15th will cost $1150, and all registrations from May 16th to June 15th will cost $1200, and finally all registrations from June 16th - July 1st will cost $1250

Payment is due by July 1st, 2010 in the form of Check for US Bank Account made payable to " Temple University" and mailed to: Dr Keith E Latham,3307 North Broad Street,Philadelphia, PA 19140 ,USA.

(includes costs of lectures, laboratory materials and supplies, access to commercial online data analysis software)

Please note that if you require a written receipt for this, you will have to notify us through email ahead of time.

Preger Molecular Embryology Course 2010 Agenda

August 8, 2010 Sunday::

4:30 PM Arrival, Welcome, Orientation, and Introductory Lecture
Classroom Fels Institute, classroom 310A, AHB building
5:00-6:00 Brief evening Lecture Keith Latham

Lectures in classrooms as indicated
Laboratory work is in the Latham lab, rooms 557,559,561 AHB

August 9 Monday: mRNA isolation and Amplification

08:00-09:00 Lecture Paula Stein "Approaches and Applications of Expression Arrays in Embryos"(310A, AHB)
09:00-09:30 Keith Latham --Overview of upcoming lab activities
09:30-12:00 Laboratory work-Ovation Embryo lysis, mRNA extraction, In Vitro Transcription (Santhi Potireddy, Namdori Mtango, Cheng-Guang Liang)
12:00-01:00 Lunch and Presentation by TU EHS Office
01:00-03:00 Continue laboratory work; discus comparisons between different methods (Namdori Mtango)
03:00-04:00 Lecture Richard Schultz " Molecular Determinants of Oocyte Quality" (310A, AHB building)

Evenings Free for dinner, informal group meetings between students

August 10 Tuesday: Array Analysis I

08:00-09:00 Lecture Zoran Obradovic" Approaches to Array Data Analysis"( 310A, AHB building)
09:00-12:00 Laboratory work-Ovation cDNA synthesis and amplification (6h ; Namdori Mtango, Cheng-Guang Liang)
12:00-01:00 Lunch
01:00-03:00 Continue laboratory work
03:00-04:00 Presentation by Agilent Technologies (Gene Spring) ( 310A, AHB building)
04:00-05:00 Lecture Christos Coutifaris "Human Reproductive Biology Molecular Frontiers" ( 310A, AHB building)
........................"From the research laboratory to clinical in vitro fertilization: Progress and Future Challenges"

Evenings Free for dinner, informal group meetings between students

August 11 Wednesday: Array Analysis II

09:00-10:00 Lecture Naoko Tanese "Deep Sequencing for Gene Expression Analysis" ( 310A, AHB)
10:00-02:00 Laboratory work -QADB lysis and RT and PCR  (Bela Patel)
02:00-03:00 Lecture Donald Baldwin "Experimental Applications of Array Technologies" ( 310A, AHB building)
03:00-04:00 Presentation by Tim Horsley (Applied Biotechnology) qRT-PCR products
04:00-05:00 Array Data Analysis demonstration(Namdori Mtango, Zhiming Han)

Evenings Free for dinner, informal group meetings between students

August 12 Thursday: ChIP TEchnologies

08:00-09:00 Lecture Nora Engel " Potential and Application of ChIP Technologies" (AHB 310A)
09:00-12:00 Laboratory work, complete In vitro Transcription and QADB Procedures (Santhi Potireddy, Bela Patel, Namdori Mtango, Cheng-Guang Liang)
12:00-1:00 Lunch
01:00-03:00 qRT-PCR ABI Prism assemble and run reactions
03:00-04:00 Presentation by Ingenuity Systems (pathway analysis) (Kresge room D)
04:00-05:00 Lecture Carmen Sapienza "Genomic Approaches to Reproductive Biology "(Kresge room D)

Evenings Free for dinner, informal group meetings between students

August 13 Friday: QADB Method

08:00-09:00 Lecture Keith Latham "QADB Method and Applications" (classroom 310A, AHB building)
09:00-12:00 Whole Genome Amplification Method (Namdori Mtango and Judy Procknow )
12:00-01:00 Lunch
01:00-01:30 Brief tour of PREGER web site (Latham lab conference room)
01:30-05:00 Laboratory work--oocyte and embryo fixation (Cheng-Guang Liang and Yong Cheng)
05:00-06:00 Lecture Keith Latham "Practical considerations in experimental design and planning for sample usage"

Evenings Free for dinner, informal group meetings between students

August 14 Saturday: Additional Lab work if needed

August 15 Sunday: Off

August 16 Monday: Proteomics

08:00-09:00 Lecture Salim Merali (AHB 310A )
09:00-12:00 Laboratory work-- demonstration of mass spec analysis
12:00-01:00 Lunch
01:00-03:00 Continue laboratory work
03:00-04:00 Lecture Zhiming Han "ABI qRT-PCR Data Analysis"(AHB 310A )
04:00-05:00 Lecture Kangpu Xu"Whole Genome Amplification and PGD"(AHB 310A )

Evenings Free for dinner, informal group meetings between students

August 17 Tuesday: Genome Manipulation

08:00-12:00 Laboratory Work: continue Whole Genome Amplification method (Namdori Mtango, Judy Procknow)
12:00-01:00 Lunch
01:00-04:00 Microsurgery demonstration, IVM/IVF demonstrations Yong Cheng
04:00-05:00 Lecture Monica Mainigi "Research in oocyte quality" ( 310A, AHB building)

Evenings Free for dinner, informal group meetings between students

August 18 Wednesday: Immunocytochemistry and Confocal Analysis

08:00-09:00 Lecture Paula Stein ( 310A, AHB building)
09:00-12:00 Laboratory work---oocyte and embryo staining
12:00-01:00 Lunch
01:00-03:00 Continue laboratory work-confocal imaging and analysis(Cheng-Guang Liang)
03:00-04:00 Presentation by B&B Microscopes/Olympus (classroom 310A, AHB building)
04:00-05:00 Lecture Catherine Racowsky "Challenges in the Human IVF Lab in 2009" (310A, AHB building)

Evenings Free for dinner, informal group meetings between students

August 19 Thursday:

09:00-10:00 Lecture Charles Chaffin "Oocyte-Follicle Interactions" (SFC building room C 4th floor)
10:00-11:00 Lecture Catherine VandeVoort "Non Human Primate Reproductive Biology"
11:00-12:00 Awarding of Certificates and departure