Course registration fee : We have special rates for early registerations. Details are below.

All registrations made by May 15th will cost $1200, and all registrations from May 16th to June 15th will cost $1250, and finally all registrations from June 16th - July 1st will cost $1300

Payment is due by July 1st, 2010 in the form of Check for US Bank Account made payable to " Temple University" and mailed to: Dr Keith E Latham,3307 North Broad Street,Philadelphia, PA 19140 ,USA.

(includes costs of lectures, laboratory materials and supplies, access to commercial online data analysis software)

Please note that if you require a written receipt for this, you will have to notify us through email ahead of time.

Preger Molecular Embryology Course 2011 Schedule (Preliminary Draft)

Lectures in classrooms as indicated
Laboratory work is in the Latham lab, rooms 557,559,561 AHB

August 7, 2011 Sunday::

4:00 PM Arrival, Welcome, Orientation,Sandwiches and Refreshments, and Introductory Lecture Keith Latham
Classroom Fels Institute, classroom 310A, AHB building

August 8 Monday: mRNA isolation and Amplification

08:00-09:00 Lecture(classroom 310A, AHB building) Paula Stein "Approaches and Applications of Expression Arrays in Embryos"
09:00-09:30 Keith Latham --comparison of RNA amplification strategies; summory of upcoming lab activities
09:30-12:00 Laboratory work- mRNA isolation amplification Embryo collection and lysis (10 min), RNA extraction (~1h 15 min), Round one First strand cDNA synthesis (~2h); (Namdori Mtango)
12:00-01:00 Lunch and Presentation by TU EHS Office
01:00-03:00 Continue laboratory work (Round one Second strand cDNA synthesis (~1h); Round one cDNA purification (~30 min); Preparation of Round one IVT reaction (~15 min); (Namdori Mtango); Meanwhile discuss comparisons between different methods and kits (Keith Latham, Namdori Mtango); Go over bioanalyzer results
03:00-04:00 Lecture (classroom 310A, AHB building) Keith Latham Molecular Determinants of Oocyte Quality
Evenings Free for dinner, informal group meetings between students

August 9 Tuesday: Array Analysis I

08:00-09:00 Lecture Zoran Obradovic" Approaches to Array Data Analysis"( 310A, AHB building)
09:00-12:00 Continue cRNA amplification, purification (~30 min); Round two First strand cDNA synthesis (~2h);  (Namdori Mtango)
12:00-01:00 Lunch
01:00-03:00Round two Second strand cDNA synthesis(~1h) Round two cDNA purification(~30 min); Preparation of Genechip IVT Labelling(~30 min) ; leave overnight.(Namdori Mtango)
03:00-04:00 Lecture (classroom 310A, AHB building) Keith Latham  QADB Method and Applications

04:00-05:00 Informal Discussion

Evenings Free for dinner, informal group meetings between students

August 10 Wednesday: Array Analysis II

09:00-11:00 Continue array methods (~35 min);
11:00-12:00 Naoko Tanese Lecture (classroom 310A, AHB building) (Namdori Mtango)Deep Sequencing Technology and Applications
012:00-01:00 Lunch
01:00-02:00 Gene Spring Demonstration (Namdori Mtango )
02:00-03:00 Donald Baldwin Experimental Applications of Array Technologies(classroom 310A, AHB building)
03:00-04:00 Applied Biotechnology  qRT-PCR products

Evenings Free for dinner, informal group meetings between students

August 11 Thursday: RT-PCR and QADB Method

08:00-09:00 qRT-PCR ABI Prism, assemble and run reaction ( Namdori Mtango)
09:00- 1:00 QADB Lysis, RT, two rounds PCR, demonstrate data quantification (Bela Patel)
01:00 -2:00 working Lunch & Lecture (lunch to be provided) (classroom 310A AHB)
Keith Latham Practical considerations in experimental design and planning for sample usage
02:00-03:00 Presentation by Christine Tavano Ingenuity Systems (pathway analysis) (310A AHB)
03:00-05:00 Informal Discussion
Evenings Free for dinner, informal group meetings between students

August 12 Friday: Oocytes and Embryo Genome Biology

09:00-12:00 Whole Genome Amplification Method(Namdori Mtango)
12:00-1:00 Lunch
01:00-02:00 Brief Tour of Preger Website(Latham lab conference room)
02:00-03:00 ABI qRT-PCR Data Analysis, ( Namdori Mtango)
03:00-04:00 oocyte and embryo fixation (Yong Cheng)

04:00-05:00 Informal Discussions

Evenings Free for dinner, informal group meetings between students

August 13 Saturday: off

August 14 Sunday: Off

August 15 Monday: Oocyte and Embryo Genome Biology and Proteomics

08:00-09:00 Lecture (AHB 310A) Nora Engel  Potential and Application of ChIP Technologies
09:00- 12:00 Proteomics Lecture and Lab(AHB 310A) Salim Mareli
12:00-01:00 Lunch
03:00-04:00 Lecture Kangpu Xu"Whole Genome Amplification and PGD"(AHB 310A )

Evenings Free for dinner, informal group meetings between students

August 16 Tuesday: Genome Manipulation

08:00- 9:00 Paula Stein (classroom 310A, AHB building)
09:00-12:00 Continue proteomics work (Namdori Mtango) and In vitro Transcription(Ed Romasko)
12:00- 1:00 working Lunch and Lecture (lunch to be provided), Spindle formation and Function Keith Latham
01:00-3:00 Laboratory work - immunofluorescence labeling of embryos, (Yong Cheng)
03:00-5:00 Confocal imaging of results (Lanping Hao )
Afternoon: Olympus Demo, Fluoview

Evenings Free for dinner, informal group meetings between students

August 17 Wednesday: Minisymposium

Oocyte Biology
8:00 Charles Chaffin
8:45 George Smith
9:30 Scott Coonrod

10:15-10:30 Coffee Break

Embryo Biology
10:15 Hugh Clarke
11:15 Richard Schultz

12:15-2:00 Lunch & Exhibits

Epigenetics
2:00 Rebecca Simmons
2:45 Rich Chaillet

3:30 Brief Discussion

4:00 Brief Graduation Ceremony

AHB 3307 North Broad Street

Room 101